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zbp1  (Boster Bio)


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    Boster Bio zbp1
    PIEZO1-mediated ferritinophagy activates the <t>Z-mtDNA-ZBP1</t> signaling axis. (A) Western blot analysis of ZBP1 expression in CEPCs treated with H 2 O 2 (100 μM) for 24 h and Yoda1 (10 μM) for 0.5, 1, 4, 12, or 24 h ( n = 3). (B) Immunofluorescence analysis of ZBP1 in CEPCs following treatment with H 2 O 2 (100 μM), Yoda1 (10 μM) or GsMTx4 (1 μM) for 24 h ( n = 3). (C) Western blot analysis of ZBP1 expression in CEPCs transfected with si-Ncoa4 or si-NC for 72 h, followed by treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (D and E) Fluorescence colocalization analysis of Z-DNA and TOMM20 ( n = 3). (F and G) Fluorescence colocalization analysis of Z-DNA and ZBP1 ( n = 3). Arrows indicate colocalization. (H-J) Western blot analysis of ZBP1 expression and immunofluorescence colocalization analysis of Z-DNA with TOMM20 in CEPCs pretreated with CsA (5 μg/mL) or EtBr (1 μg/mL) for 12 h, followed by co-treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (K) Immunohistochemistry analysis of ZBP1 expression in LSI model following 3-month treatment with Yoda1 (3 mg/kg/w) and CsA (20 μg/kg/w) ( n = 4). (L and M) Immunohistochemistry analysis of ZBP1 expression in Tail-looping model after 8 weeks of intervention with AAV-GFP , AAV-Ncoa4 KD or AAV-Ncoa4 OE ( n = 4).
    Zbp1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+zbp1/Anti-DAI%2FZbp1+Antibody+Picoband/pmc13618854-408-76-77
    Average 94 stars, based on 5 article reviews
    zbp1 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via a PIEZO1-NCOA4-ZBP1 axis"

    Article Title: Mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via a PIEZO1-NCOA4-ZBP1 axis

    Journal: Autophagy

    doi: 10.1080/15548627.2026.2677183

    PIEZO1-mediated ferritinophagy activates the Z-mtDNA-ZBP1 signaling axis. (A) Western blot analysis of ZBP1 expression in CEPCs treated with H 2 O 2 (100 μM) for 24 h and Yoda1 (10 μM) for 0.5, 1, 4, 12, or 24 h ( n = 3). (B) Immunofluorescence analysis of ZBP1 in CEPCs following treatment with H 2 O 2 (100 μM), Yoda1 (10 μM) or GsMTx4 (1 μM) for 24 h ( n = 3). (C) Western blot analysis of ZBP1 expression in CEPCs transfected with si-Ncoa4 or si-NC for 72 h, followed by treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (D and E) Fluorescence colocalization analysis of Z-DNA and TOMM20 ( n = 3). (F and G) Fluorescence colocalization analysis of Z-DNA and ZBP1 ( n = 3). Arrows indicate colocalization. (H-J) Western blot analysis of ZBP1 expression and immunofluorescence colocalization analysis of Z-DNA with TOMM20 in CEPCs pretreated with CsA (5 μg/mL) or EtBr (1 μg/mL) for 12 h, followed by co-treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (K) Immunohistochemistry analysis of ZBP1 expression in LSI model following 3-month treatment with Yoda1 (3 mg/kg/w) and CsA (20 μg/kg/w) ( n = 4). (L and M) Immunohistochemistry analysis of ZBP1 expression in Tail-looping model after 8 weeks of intervention with AAV-GFP , AAV-Ncoa4 KD or AAV-Ncoa4 OE ( n = 4).
    Figure Legend Snippet: PIEZO1-mediated ferritinophagy activates the Z-mtDNA-ZBP1 signaling axis. (A) Western blot analysis of ZBP1 expression in CEPCs treated with H 2 O 2 (100 μM) for 24 h and Yoda1 (10 μM) for 0.5, 1, 4, 12, or 24 h ( n = 3). (B) Immunofluorescence analysis of ZBP1 in CEPCs following treatment with H 2 O 2 (100 μM), Yoda1 (10 μM) or GsMTx4 (1 μM) for 24 h ( n = 3). (C) Western blot analysis of ZBP1 expression in CEPCs transfected with si-Ncoa4 or si-NC for 72 h, followed by treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (D and E) Fluorescence colocalization analysis of Z-DNA and TOMM20 ( n = 3). (F and G) Fluorescence colocalization analysis of Z-DNA and ZBP1 ( n = 3). Arrows indicate colocalization. (H-J) Western blot analysis of ZBP1 expression and immunofluorescence colocalization analysis of Z-DNA with TOMM20 in CEPCs pretreated with CsA (5 μg/mL) or EtBr (1 μg/mL) for 12 h, followed by co-treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (K) Immunohistochemistry analysis of ZBP1 expression in LSI model following 3-month treatment with Yoda1 (3 mg/kg/w) and CsA (20 μg/kg/w) ( n = 4). (L and M) Immunohistochemistry analysis of ZBP1 expression in Tail-looping model after 8 weeks of intervention with AAV-GFP , AAV-Ncoa4 KD or AAV-Ncoa4 OE ( n = 4).

    Techniques Used: Western Blot, Analysis, Expressing, Immunofluorescence, Transfection, Fluorescence, Immunohistochemistry

    ZBP1 activates the NFKB pathway, thereby exacerbating CEP osteochondritis and degeneration. CEPCs were transfected with si-Zbp1 or si-NC for 72 h following H 2 O 2 (100 μM) and Yoda1 (10 μM) induction for 24 h. The intervertebral spaces of the Tail-looping mouse model were injected with AAV-Zbp1 KD or AAV-GFP and evaluated after 2 months. (A) Western blot analysis of NFKB and p-NFKB expression in CEPCs ( n = 3). (B and E) Immunofluorescence analysis of Mito-ROS levels in CEPCs ( n = 3). (C, D, F and G) Immunohistochemical analysis of IL1B, IL6 and TNF expression in CEP ( n = 4). (H and I) Western blot analysis of ECM metabolism markers (COL2, SOX9, COL10A1, RUNX2, MMP13, MMP3) in CEPCs ( n = 3). (J and K) Immunofluorescence analysis of COL2 and COL10A1 expression in CEP ( n = 4). (L) Schematic diagram of mechanical stress-induced iron homeostasis disorder and degeneration in the CEPCs.
    Figure Legend Snippet: ZBP1 activates the NFKB pathway, thereby exacerbating CEP osteochondritis and degeneration. CEPCs were transfected with si-Zbp1 or si-NC for 72 h following H 2 O 2 (100 μM) and Yoda1 (10 μM) induction for 24 h. The intervertebral spaces of the Tail-looping mouse model were injected with AAV-Zbp1 KD or AAV-GFP and evaluated after 2 months. (A) Western blot analysis of NFKB and p-NFKB expression in CEPCs ( n = 3). (B and E) Immunofluorescence analysis of Mito-ROS levels in CEPCs ( n = 3). (C, D, F and G) Immunohistochemical analysis of IL1B, IL6 and TNF expression in CEP ( n = 4). (H and I) Western blot analysis of ECM metabolism markers (COL2, SOX9, COL10A1, RUNX2, MMP13, MMP3) in CEPCs ( n = 3). (J and K) Immunofluorescence analysis of COL2 and COL10A1 expression in CEP ( n = 4). (L) Schematic diagram of mechanical stress-induced iron homeostasis disorder and degeneration in the CEPCs.

    Techniques Used: Transfection, Injection, Western Blot, Analysis, Expressing, Immunofluorescence, Immunohistochemical staining

    Related Articles

    Blocking Assay:

    Article Title: Identification of PANoptosis hub genes driving immune activation and tubulointerstitial injury in diabetic kidney disease by integrative bioinformatics and machine learning
    Article Snippet: .. After blocking, membranes were incubated with primary antibodies at 4 °C overnight (anti-NGAL, Abcam; anti-caspase-1, Abmart; anti-GSDMD, Abcam; anti-GAPDH, Proteintech; anti-ZBP1, Proteintech; anti-fibronectin/FN, Proteintech; anti-α-SMA, Proteintech; anti-Kim-1, Boster; anti-YWHAH, Solarbio; anti-PRKACB, Solarbio; anti-PSMB9, Solarbio; anti-GZMA, Proteintech; anti-caspase-3, Cell Signaling Technology; anti-FAS, Abmart; anti-MLKL, abcam), followed by appropriate HRP-conjugated secondary antibodies. ..

    Incubation:

    Article Title: Identification of PANoptosis hub genes driving immune activation and tubulointerstitial injury in diabetic kidney disease by integrative bioinformatics and machine learning
    Article Snippet: .. After blocking, membranes were incubated with primary antibodies at 4 °C overnight (anti-NGAL, Abcam; anti-caspase-1, Abmart; anti-GSDMD, Abcam; anti-GAPDH, Proteintech; anti-ZBP1, Proteintech; anti-fibronectin/FN, Proteintech; anti-α-SMA, Proteintech; anti-Kim-1, Boster; anti-YWHAH, Solarbio; anti-PRKACB, Solarbio; anti-PSMB9, Solarbio; anti-GZMA, Proteintech; anti-caspase-3, Cell Signaling Technology; anti-FAS, Abmart; anti-MLKL, abcam), followed by appropriate HRP-conjugated secondary antibodies. ..



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    Image Search Results


    PIEZO1-mediated ferritinophagy activates the Z-mtDNA-ZBP1 signaling axis. (A) Western blot analysis of ZBP1 expression in CEPCs treated with H 2 O 2 (100 μM) for 24 h and Yoda1 (10 μM) for 0.5, 1, 4, 12, or 24 h ( n = 3). (B) Immunofluorescence analysis of ZBP1 in CEPCs following treatment with H 2 O 2 (100 μM), Yoda1 (10 μM) or GsMTx4 (1 μM) for 24 h ( n = 3). (C) Western blot analysis of ZBP1 expression in CEPCs transfected with si-Ncoa4 or si-NC for 72 h, followed by treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (D and E) Fluorescence colocalization analysis of Z-DNA and TOMM20 ( n = 3). (F and G) Fluorescence colocalization analysis of Z-DNA and ZBP1 ( n = 3). Arrows indicate colocalization. (H-J) Western blot analysis of ZBP1 expression and immunofluorescence colocalization analysis of Z-DNA with TOMM20 in CEPCs pretreated with CsA (5 μg/mL) or EtBr (1 μg/mL) for 12 h, followed by co-treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (K) Immunohistochemistry analysis of ZBP1 expression in LSI model following 3-month treatment with Yoda1 (3 mg/kg/w) and CsA (20 μg/kg/w) ( n = 4). (L and M) Immunohistochemistry analysis of ZBP1 expression in Tail-looping model after 8 weeks of intervention with AAV-GFP , AAV-Ncoa4 KD or AAV-Ncoa4 OE ( n = 4).

    Journal: Autophagy

    Article Title: Mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via a PIEZO1-NCOA4-ZBP1 axis

    doi: 10.1080/15548627.2026.2677183

    Figure Lengend Snippet: PIEZO1-mediated ferritinophagy activates the Z-mtDNA-ZBP1 signaling axis. (A) Western blot analysis of ZBP1 expression in CEPCs treated with H 2 O 2 (100 μM) for 24 h and Yoda1 (10 μM) for 0.5, 1, 4, 12, or 24 h ( n = 3). (B) Immunofluorescence analysis of ZBP1 in CEPCs following treatment with H 2 O 2 (100 μM), Yoda1 (10 μM) or GsMTx4 (1 μM) for 24 h ( n = 3). (C) Western blot analysis of ZBP1 expression in CEPCs transfected with si-Ncoa4 or si-NC for 72 h, followed by treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (D and E) Fluorescence colocalization analysis of Z-DNA and TOMM20 ( n = 3). (F and G) Fluorescence colocalization analysis of Z-DNA and ZBP1 ( n = 3). Arrows indicate colocalization. (H-J) Western blot analysis of ZBP1 expression and immunofluorescence colocalization analysis of Z-DNA with TOMM20 in CEPCs pretreated with CsA (5 μg/mL) or EtBr (1 μg/mL) for 12 h, followed by co-treatment with H 2 O 2 (100 μM) or Yoda1 (10 μM) for 24 h ( n = 3). (K) Immunohistochemistry analysis of ZBP1 expression in LSI model following 3-month treatment with Yoda1 (3 mg/kg/w) and CsA (20 μg/kg/w) ( n = 4). (L and M) Immunohistochemistry analysis of ZBP1 expression in Tail-looping model after 8 weeks of intervention with AAV-GFP , AAV-Ncoa4 KD or AAV-Ncoa4 OE ( n = 4).

    Article Snippet: Chondrocytes were fixed with 4% PFA (Servicebio, G1101) for 30 min, followed by permeabilization utilizing 0.1% Triton X-100 (Solarbio, T8200) for 10 min and then blocking with 5% BSA (Biosharp, BS114) for 1 h. Subsequently, the cells were incubated with the primary antibodies against NCOA4 (Abclonal, A5695), FTH (Boster, BM4487), LC3B (Cell Signaling Technology, 83506), LAMP1 (Cell Signaling Technology, 99437), COL2/collagen II (Proteintech, 28459-1-AP), COL10A1/collagen X (Boster, BA2023), YAP1 (Cell Signaling Technology, 14074), TOMM20 (Abcam, ab186735), ZBP1 (Boster, A04739-3) and Z-DNA (Novus, NB100-749) at 4°C overnight.

    Techniques: Western Blot, Analysis, Expressing, Immunofluorescence, Transfection, Fluorescence, Immunohistochemistry

    ZBP1 activates the NFKB pathway, thereby exacerbating CEP osteochondritis and degeneration. CEPCs were transfected with si-Zbp1 or si-NC for 72 h following H 2 O 2 (100 μM) and Yoda1 (10 μM) induction for 24 h. The intervertebral spaces of the Tail-looping mouse model were injected with AAV-Zbp1 KD or AAV-GFP and evaluated after 2 months. (A) Western blot analysis of NFKB and p-NFKB expression in CEPCs ( n = 3). (B and E) Immunofluorescence analysis of Mito-ROS levels in CEPCs ( n = 3). (C, D, F and G) Immunohistochemical analysis of IL1B, IL6 and TNF expression in CEP ( n = 4). (H and I) Western blot analysis of ECM metabolism markers (COL2, SOX9, COL10A1, RUNX2, MMP13, MMP3) in CEPCs ( n = 3). (J and K) Immunofluorescence analysis of COL2 and COL10A1 expression in CEP ( n = 4). (L) Schematic diagram of mechanical stress-induced iron homeostasis disorder and degeneration in the CEPCs.

    Journal: Autophagy

    Article Title: Mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via a PIEZO1-NCOA4-ZBP1 axis

    doi: 10.1080/15548627.2026.2677183

    Figure Lengend Snippet: ZBP1 activates the NFKB pathway, thereby exacerbating CEP osteochondritis and degeneration. CEPCs were transfected with si-Zbp1 or si-NC for 72 h following H 2 O 2 (100 μM) and Yoda1 (10 μM) induction for 24 h. The intervertebral spaces of the Tail-looping mouse model were injected with AAV-Zbp1 KD or AAV-GFP and evaluated after 2 months. (A) Western blot analysis of NFKB and p-NFKB expression in CEPCs ( n = 3). (B and E) Immunofluorescence analysis of Mito-ROS levels in CEPCs ( n = 3). (C, D, F and G) Immunohistochemical analysis of IL1B, IL6 and TNF expression in CEP ( n = 4). (H and I) Western blot analysis of ECM metabolism markers (COL2, SOX9, COL10A1, RUNX2, MMP13, MMP3) in CEPCs ( n = 3). (J and K) Immunofluorescence analysis of COL2 and COL10A1 expression in CEP ( n = 4). (L) Schematic diagram of mechanical stress-induced iron homeostasis disorder and degeneration in the CEPCs.

    Article Snippet: Chondrocytes were fixed with 4% PFA (Servicebio, G1101) for 30 min, followed by permeabilization utilizing 0.1% Triton X-100 (Solarbio, T8200) for 10 min and then blocking with 5% BSA (Biosharp, BS114) for 1 h. Subsequently, the cells were incubated with the primary antibodies against NCOA4 (Abclonal, A5695), FTH (Boster, BM4487), LC3B (Cell Signaling Technology, 83506), LAMP1 (Cell Signaling Technology, 99437), COL2/collagen II (Proteintech, 28459-1-AP), COL10A1/collagen X (Boster, BA2023), YAP1 (Cell Signaling Technology, 14074), TOMM20 (Abcam, ab186735), ZBP1 (Boster, A04739-3) and Z-DNA (Novus, NB100-749) at 4°C overnight.

    Techniques: Transfection, Injection, Western Blot, Analysis, Expressing, Immunofluorescence, Immunohistochemical staining